Coupling of Escherichia coli lipopolysaccharide to epoxy-activated Sepharose 6B
نویسندگان
چکیده
منابع مشابه
Escherichia coli Lipopolysaccharide
CD-1 mice immunized with sheep red blood cells (SRBC) or Escherichia coli lipopolysaccharide (LPS), developed splenic plaque-forming cells (PFC) producing low-molecular-weight antibody; these cells were detected by means of purified rabbit antisera to mouse 'y', 'Y2a, and 2b immunoglobulins. In contrast to SRBC, the primary -y, response to LPS was absent and 8,2a and 'y2b PFC were detected irre...
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Escherichia coli K-12 varkappa971 was crossed with a smooth Salmonella typhimurium donor, HfrK6, which transfers early the ilv-linked rfa region determining lipopolysaccharide (LPS) core structure. Two ilv(+) hybrids differing in their response to the LPS-specific phages FO and C21 were then crossed with S. typhimurium HfrK9, which transfers early the rfb gene cluster determining O repeat unit ...
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In the title compounds, C21H30O4, (I), and C23H34O4, (II), respectively, which are valuable intermediates in the synthesis of important steroid derivatives, rings A and B are cis(5 ,10 )-fused. The two molecules have similar conformations of rings A, B and C. The presence of the 5 ,6 -epoxide group induces a significant twist of the steroid nucleus and a strong flattening of the B ring. The dif...
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1. Heparin, heparan sulphate, chondroitin sulphate and dermatan sulphate were covalently attached to beads of agarose activated by cyanogen bromide. The bond is probably mediated by the amino group of a serine or peptide residue at the reducing end of the polysaccharide chain. 2. The uptake of glycosaminoglycan during the coupling procedure is about 0.9mg/ml of wet gel. However, direct analysis...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1978
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.20.3.867-868.1978